biochanin a modulates cell viability, invasion, and growth promoting signaling pathways in her-2-positive breast cancer cellsbiochanin调节细胞生存能力、入侵和增长促进her-2-positive乳腺癌细胞信号通路.pdfVIP

Hindawi Publishing Corporation Journal of Oncology Volume 2009, Article ID 121458, 10 pages doi:10.1155/2009/121458 Research Article Biochanin A Modulates Cell Viability, Invasion, and Growth Promoting Signaling Pathways in HER-2-Positive Breast Cancer Cells Vikas Sehdev, James C. K. Lai, and Alok Bhushan Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy Idaho, Biomedical Research Institute, Idaho State University, Pocatello, ID 83209, USA Correspondence should be addressed to Alok Bhushan, abhushan@ Received 24 June 2009; Revised 3 November 2009; Accepted 10 November 2009 Recommended by Bruno Vincenzi Overexpression of HER-2 receptor is associated with poor prognosis and aggressive forms of breast cancer. Scientific literature indicates a preventive role of isoflavones in cancer. Since activation of HER-2 receptor initiates growth-promoting events in cancer cells, we studied the effect of biochanin A (an isoflavone) on associated signaling events like receptor activation, downstream signaling, and invasive pathways. HER-2-positive SK-BR-3 breast cancer cells, MCF-10A normal breast epithelial cells, and NIH- 3T3 normal fibroblast cells were treated with biochanin A (2–100 μM) for 72 hours. Subsequently cell viability assay, western blotting and zymography were carried out. The data indicate that biochanin A inhibits cell viability, signaling pathways, and invasive enzyme expression and activity in SK-BR-3 cancer cells. Biochanin A did not inhibit MCF-10A and NIH-3T3 cell viability. Therefore, biochanin A could be a unique natural anticancer agent which can selectively target cancer cells and inhibit multiple signaling pathways in HER-2-positive breast cancer cells. Copyright © 2009 Vikas Sehdev et al.