cellular profiles in peripheral blood accompanying particular asthmatic response types细胞在外周血附带特殊类型的哮喘反应.pdfVIP

International Journal of Clinical Medicine, 2012, 3, 485-497 485 / 10.4236/ijcm.2012.36088 Published Online November 2012 (http://www.SciRP.org/journal/ijcm) Cellular Profiles in Peripheral Blood Accompanying Particular Asthmatic Response Types Zdenek Pelikan Allergy Research Foundation, Breda, The Netherlands. Email: zpelikan@casema.nl Received July 23rd, 2012; revised August 26th, 2012; accepted September 13th, 2012 ABSTRACT Background: Patients with allergic bronchial asthma develop various asthmatic response types to bronchial challenge with allergen, such as immediate (IAR), late (LAR), dual late (DLAR) or delayed (DYAR), displaying different clinical, immunologic and pharmacologic features. This study deals with count changes of particular blood cells accompanying the IAR, LAR and DYAR. Methods: In 63 patients developing 22 IAR, 26 LAR and 15 DYAR, the repeated allergen challenges were supplemented with recording of blood cell counts, Th /Th ratio, leukotrines B (LTB ) and C (LTC ), 1 2 4 4 4 4 eosinophil cationic protein (ECP), myeloperoxidase (MPO), and histamine in blood, and intracellular IFN-γ and IL-4 in peripheral blood mononuclear cells. Results: The IAR was accompanied by increased eosinophil and basophil counts, increased serum concentrations of histamine, LTC and ECP, decreased Th /Th ratio in favour of Th cells, and in- 4 1 2 2 creased intracellular IL-4. The LAR was associated with increased eosinophil and neutrophil counts, increased serum concentrations of LTC and LTB , unchanged Th /Th ratio, and increased intracellular IL-4. The DYAR was accom- 4 4