cellulosic ethanol production by recombinant cellulolytic bacteria harbouring pdc and adh ii genes of zymomonas mobilis重组纤维素分解细菌纤维素乙醇生产的发酵单胞菌属mobilis窝藏pdc和adh ii基因.pdfVIP

Hindawi Publishing Corporation Biotechnology Research International Volume 2012, Article ID 817549, 8 pages doi:10.1155/2012/817549 Research Article Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis P. Sobana Piriya, P. Thirumalai Vasan, V. S. Padma, U. Vidhyadevi, K. Archana, and S. John Vennison Department of Biotechnology, Anna University of Technology, Tamil Nadu, Tiruchirappalli 620024, India Correspondence should be addressed to S. John Vennison, johnvennison36@ Received 26 March 2012; Accepted 12 June 2012 Academic Editor: Triantafyllos Roukas Copyright © 2012 P. Sobana Piriya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The ethanol fermenting genes such as pyruvate decarboxylase (pdc ) and alcohol dehydrogenase II (adh II) were cloned from Zymomonas mobilis and transformed into three different cellulolytic bacteria, namely Enterobacter cloacae JV, Proteus mirabilis JV and Erwinia chrysanthemi and their cellulosic ethanol production capability was studied. Recombinant E. cloacae JV was found to produce 4.5% and 3.5% (v/v) ethanol, respectively, when CMC and 4% NaOH pretreated bagasse were used as substrates, whereas recombinant P. mirabilis and E. chrysanthemi with the same substrates could only produce 4%, 3.5%, 1%, and 1.5 % of ethanol, respectively. The recombinant E. cloacae strain produced twofold higher percentage of ethanol than the wild type. The recombinant E. cloacae strain could be improved further by increasing its ethanol tolerance capability through media optimization and also by comb