characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic stem cells特征的间充质干细胞线区分骨头和提供支持鼠标和人类造血干细胞利基市场.pdfVIP

Vol.2, No.1, 5-14 (2012) Stem Cell Discovery /10.4236/scd.2012.21002 Characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic stem cells 1 2 3 4 Sonal R. Tuljapurkar , John D. Jackson , Susan K. Brusnahan , Barbara J. O’Kane , John G. Sharp1* 1Departments of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, USA 2Pathology and Microbiology, University of Nebraska Medical Center, Omaha, USA 3Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, USA 4Creighton University Medical Center, Omaha, USA; *Corresponding Author: jsharp@ Received 7 October 2011; revised 10 November 2011; accepted 21 November 2011 ABSTRACT were used therapeutically for skeletal problems [2] and gene therapy [3,4] multiple additional therapeutic appli- Identification of mouse cell lines with properties cations have been evaluated, including promotion of he- of primary multipotential mesenchymal stromal matopoietic recovery in stem cell transplant recipients [5,6] cells (MSC) is required to facilitate the use of severe, acute graft-versus-host disease [7], autoimmune mouse models for evaluation of mechanisms in diseases [8], muscle repair [9], skin healing [10], intes- bone formation, hematopoiesis and cellular the- tine healing following irradiation [11], stroke [12], myo- rapies for regenerative medicine. Primary mur- cardial ischemia [1