cytochrome p450 induction and gene expression in channel catfish (ictalurus punctatus) following wastewater treatment plant effluent exposure in field and laboratory settings细胞色素p450感应和基因表达在通道鲶鱼(ictalurus毛虫)污水处理厂污水暴露在野外和实验室设置.pdfVIP

Journal of Environmental Protection, 2010, 1, 362-373 doi:10.4236/jep.2010.14042 Published Online December 2010 (http://www.SciRP.org/journal/jep) Cytochrome P450 Induction and Gene Expression in Channel Catfish (Ictalurus punctatus) Following Wastewater Treatment Plant Effluent Exposure in Field and Laboratory Settings 1 1 1 1 2 Alicia Whatley *, In Ki Cho , Christi Magrath , Paul M. Stewart , Robert W. Li 1Department of Biological and Environmental Sciences, Troy University, Troy, USA; 2Bovine Functional Genomics Laboratory, USDA, Beltsville, USA. Email: awhatley@. Received July 16th, 2010; revised August 26th, 2010; accepted August 30th, 2010. ABSTRACT The objectives of this study were as follows: 1) to establish a baseline ethoxyresorufin-O-deethylase (EROD) activity level in channel catfish (Ictalurus punctatus), 2) to assess changes in induction of cytochrome P450 enzyme in channel catfish following exposure to creek water at the discharge point from the Troy (Alabama) Wastewater Treatment Plant (TWWTP) compared to upstream samples from Walnut Creek, 3) to compare EROD activity in populations maintained in laboratory and field settings, and 4) to quantify cytochrome P450 gene expression. Enzyme activity was measured fluorometrically and CYP 1 gene expression was analyzed by quantitative real-time reverse transcription polymerase chain reaction. A mean EROD baseline was established at 0.03 nmol/min/µg of protein. The overall mean field effluent (TF) EROD had a significant 5-fold increase over field upstream (UF) exposed catfish; and overall mean laboratory effluent (TL) exposed catfish EROD had a significant 1.8-fold increase over laboratory upstream (UL) exposed catfish. Field exposures generally showed more robust enzyme induction over laboratory exposures on all sampling days. Ex- pression