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cytotoxic and inhibitory activity of ceramide on cancer cell lines细胞毒性和神经酰胺抑制活动癌症细胞系
J. Biomedical Science and Engineering, 2010, 3, 892-899 JBiSE
doi:10.4236/jbise.2010.39119 Published Online September 2010 (http://www.SciRP.org/journal/jbise/).
Cytotoxic and inhibitory activity of ceramide on cancer cell
lines
Muthana Ibrahim Maleek
Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.
Email: fadhilsalih@
Received 21 January 2010; revised 15 February 2010; accepted 15 February 2010.
ABSTRACT solid tumors and hematopoietic neoplasms. However, im-
portant obstacles were encountered in the use of chemo-
The inhibitory activity of ceramide on cancer cells therapy that included toxicity to the normal tissues of the
was evaluated and its cytotoxicity on different cancer body and the presence of mutations that confer resis-
cell lines was measured. Ceramide was separated fro- tance to these chemotherapeutic agents [5]. Therefore,
m bovine brain and spinal cord, and extracted by or- cancer patients as well as many physicians began to re-
ganic solvents. The crude extract was purified by us- quest natural products for treatment due to their multiple
ing silicic acid column. Detection and identification of effects in treating the disease, relieving patient’s symp-
purified extract were carried out by using three ass- toms in addition to improved safety and lower cost [6].
ays: visualization, spectrophotometry and infrared. Natural products have long been a fertile source of cure
Cytotoxic effect of different concentrations (7, 15, 30 for cancer, which is projected to become the major
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