development of efficient fermentation process at bioreactor level by taguchis orthogonal array methodology for enhanced dextransucrase production from weissella confusa cab3发展高效发酵过程生物反应器水平由田口正交阵列的方法增强dextransucrase生产从weissella confusa cab3.pdfVIP

Advances in Microbiology, 2012, 2, 277-283 /10.4236/aim.2012.23033 Published Online September 2012 (http://www.SciRP.org/journal/aim) Development of Efficient Fermentation Process at Bioreactor Level by Taguchi’s Orthogonal Array Methodology for Enhanced Dextransucrase Production from Weissella confusa Cab3 * Shraddha Shukla, Arun Goyal Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, India * Email: arungoyl@iitg.ernet.in Received April 20, 2012; revised May 19, 2012; accepted May 28, 2012 ABSTRACT The influence of medium ingredients on extracellular dextransucrase production by a new bacterial strain Weissella confusa Cab3 (Genbank Accession Number JX649223) was evaluated using fractional factorial design of Taguchi’s orthogonal array. Four metabolism influencing factors viz. sucrose, yeast extract, K HPO and Tween80 were selected 2 4 to optimize dextransucrase production by W. confusa Cab3 using fractional factorial design of Taguchi methodology. Based on the influence of interaction components of fermentation, least significant factors of individual level have higher interaction severity index and vice versa for enzyme production from Weissella confusa Cab3. Sucrose and yeast extract were found to be the most significant factors which positively influenced the dextransucrase production. The optimized medium composition consisted of sucrose—5%;