directly immobilized dna sensor for label-free detection of herpes virus直接固定化dna传感器label-free疱疹病毒的检测.pdfVIP

Vol.2, No.6, 374-379 (2009) JBiSE doi:10.4236/jbise.2009.26054 Directly immobilized DNA sensor for label-free detection of herpes virus Phuong Dinh Tam1,2 2 3 , Mai Anh Tuan , Nguyen Duc Chien 1Hanoi Advanced School of Science and Technology, Hanoi University of Technology, Hanoi, Vietnam; 2International Training Insti- tute for Materials Science, Hanoi University of Technology, Hanoi, Vietnam; 3Institute of Engineering Physics, Hanoi University of Technology, Hanoi, Vietnam. Email: tampd-hast@.vn Received 24 March 2008; revised 12 May 2009; accepted 29 June 2009. ABSTRACT development of a cheap, reliable device allowing rapid detection is always the challenge for scientists and engi- This paper reports the direct immobilization of neers. In this context, DNA sensor based on electro- deoxyribonucleic acid (DNA) sequences of Her- chemical detection is one of the feasible and promising pes simplex virus (5’–AT CAC CGA CCC GGA tools. GAG GGA C–3’) on the surface of DNA sensor We reported, in this paper, the direct co-immobiliza- by using the cyclic voltammetric method with tion of DNA sequence of Herpes simplex virus and the presence of pyrrole. The potential was polypyrrole onto the surface of a sensor by cyclic volt- scanned from –0.7 volt to + 0.6 volt, the scan- ammetry to determine the herpes DNA target sequence ning rate was at 100mV/s. This kind of DNA