原创力文档dna barcoding of ricinus communis from different geographical origin by using chloroplast matk and internal transcribed spacersdna条码技术来自不同地理来源的萝藦利用叶绿体matk和内部转录间隔器.pdfVIP
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dna barcoding of ricinus communis from different geographical origin by using chloroplast matk and internal transcribed spacersdna条码技术来自不同地理来源的萝藦利用叶绿体matk和内部转录间隔器
American Journal of Plant Sciences, 2012, 3, 1304-1310
/10.4236/ajps.2012.39157 Published Online September 2012 (http://www.SciRP.org/journal/ajps)
DNA Barcoding of Ricinus communis from Different
Geographical Origin by Using Chloroplast matK and
Internal Transcribed Spacers
1,2 1,3 1 1
Mohamed Enan , Nael Fawzi , Mohammad Al-Deeb , Khaled Amiri
1Department of Biology, UAE University, Al-Ain, UAE; 2Agricultural Genetic Engineering Research Institute (AGERI), Agricul-
tural Research Center (ARC), Giza, Egypt; 3Flora and Phyto-Taxonomy Research Department, Horticultural Research Institute, Ag-
ricultural Research Center, Giza, Egypt.
Email: mohamed.enan@uaeu.ac.ae
th rd st
Received June 27 , 2012; revised July 23 , 2012; accepted August 1 , 2012
ABSTRACT
Ricinus communis have attracted considerable attention because of its specific industrial and pharmacological activities.
DNA barcodes can be used as reliable tools to facilitate the identification of medicinal plants for the safe use, quality
control and forensic investigation. In this study, the differential identification of eight accessions of R. communis was
investigated through DNA sequence analysis of two candidate DNA barcodes. The nucleotide sequence of internal
transcribed spacers (ITS2) and chloroplast maturase gene (matK) have been determined to construct the phylogenetic
tree. The phylogenetic relationships of accessions based on the nrITS2 region and partial matK region showed that all
accessions in this study were related to three geographical origins. Based on sequence alignment and phylogenetic ana-
lyses we concluded that the ITS2 sequences can distinguish R. communis accessions from different geographical distri-
butions.
Keywords: DNA Barcoding; Internal Transcribed
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