dna damage in hemocytes of schistocerca gregaria (orthoptera acrididae) exposed to contaminated food with cadmium and leaddna损伤通过姬氏schistocerca gregaria(直翅目acrididae)与镉和铅暴露于受污染的食物.pdfVIP

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dna damage in hemocytes of schistocerca gregaria (orthoptera acrididae) exposed to contaminated food with cadmium and leaddna损伤通过姬氏schistocerca gregaria(直翅目acrididae)与镉和铅暴露于受污染的食物.pdf

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dna damage in hemocytes of schistocerca gregaria (orthoptera acrididae) exposed to contaminated food with cadmium and leaddna损伤通过姬氏schistocerca gregaria(直翅目acrididae)与镉和铅暴露于受污染的食物

Vol.2, No.4, 292-297 (2010) Natural Science /10.4236/ns.2010.24037 DNA damage in hemocytes of Schistocerca gregaria (Orthoptera: Acrididae) exposed to contaminated food with cadmium and lead 1* 1 2 1 Hesham A. Yousef , Amira Afify , Hany M. Hasan , Afaf Abdel Meguid 1Entomology Department, Faculty of Science, Cairo University, Cairo, Egypt; heshamyousef.eg@ 2Agriculture Research Center, Ministry of Agriculture, Cairo, Egypt Received 9 December 2009; Revised 4 January 2010; accepted 5 February 2010. ABSTRACT and the renal system [11]. Changes in the cell genome caused by genotoxic We measured in a comet assay the damage of agents leading to mutations and possibly tumor forma- DNA in the hemocytes of various stages of the tion are some of the lethal or sub-lethal effects induced grasshopper Schistocerca gregaria after ex- by a complex mixture of pollutants. Among recently posing them to various doses of Cd and Pb in used methods to identify DNA damage is the comet as- the food. The mechanisms of Cd and Pb toxicity say (SCGE-single cell gel electrophoresis). The comet for grasshopper are discussed. The accumula- assay provides a rapid, sensitive, and inexpensive tion of heavy metals and stage of the insect may method to detect DNA strand breaks in individual eu- play important roles in causing the DNA damage. karyotic cel