drosophila melanogaster selection for survival of bacillus cereus infection life history trait indirect responses黑腹果蝇的生存选择蜡样芽胞杆菌感染的生活史特征的间接反应.pdfVIP

Hindawi Publishing Corporation International Journal of Evolutionary Biology Volume 2012, Article ID 935970, 12 pages doi:10.1155/2012/935970 Research Article Drosophila melanogaster Selection for Survival of Bacillus cereus Infection: Life History Trait Indirect Responses Junjie Ma,1 Andrew K. Benson,1 Stephen D. Kachman,2 Zhen Hu,3 and Lawrence G. Harshman3 1 Department of Food Science and Technology, University of Nebraska Lincoln, Lincoln, NE 68583, USA 2 Department of Statistics, University of Nebraska Lincoln, Lincoln, NE 68583, USA 3 School of Biological Sciences, University of Nebraska Lincoln, Lincoln, NE 68588, USA Correspondence should be addressed to Lawrence G. Harshman, lharshman1@ Received 24 April 2012; Revised 26 July 2012; Accepted 16 August 2012 Academic Editor: Alberto Civetta Copyright © 2012 Junjie Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To study evolved resistance/tolerance in an insect model, we carried out an experimental evolution study using D. melanogaster and the opportunistic pathogen B. cereus as the agent of selection. The selected lines evolved a 3.0- to 3.3-log increase in the concentration of spores required for 50% mortality after 18–24 generations of selection. In the absence of any treatment, selected lines evolved an increase in egg production and delayed development time. The latter response could be interpreted as a cost of evolution. Alternatively, delayed development might have been a target of selection resulting in increased adult fat body function including production of antimicrobial peptides, and, incidentally, yolk production for