effect of recombinant human erythropoietin on survivin expression in brain tissues after traumatic brain injury in rats重组人促红细胞生成素对生存素表达的影响在大鼠创伤性脑损伤后大脑组织.pdfVIP

Surgical Science, 2012, 3, 266-270 /10.4236/ss.2012.35053 Published Online May 2012 (http://www.SciRP.org/journal/ss) Effect of Recombinant Human Erythropoietin on Survivin Expression in Brain Tissues after Traumatic Brain Injury in Rats Peng Lei, Longfeng Peng, Xingchao Zhang Department of Neurosurgery, Lanzhou General Hospital, Lanzhou, China Email: leipp2003@ Received January 8, 2011; revised December 17, 2011; accepted January 3, 2012 ABSTRACT Objective: To explore the regulative effect on Survivin of r-HuEPO after traumatic brain injury (TBI) in rats, and un- derstand the neuroprotection mechanisms of r-HuEPO. Methods: Seventy-eight adult Wistar rats were randomly di- vided into sham operation group (n = 6), TBI group (n = 36) and r-HuEPO group (n = 36). The experimental TBI model was created by Feeney’s method. Samples were obtained after injury for measuring apoptosis of cells by Epics XL Flow Cytometer. Immunochemical method was performed for inspection of expressions of Survivin and NF-κB proteins. Re- sults: Compared to the sham group, the number of apoptotic cells and Survivin, NF-κB immunopositive cells was sig- nificantly increased in the injured brain after TBI (P 0.01). R-HuEPO significantly increased the expression of sur- vivin and NF-κB, but decreased the apoptotic rates. Conclusion: Increased expression of NF-κB by r-HuEPO may play important role in regulating Survivin level, inhibiting neuronal apoptosis in cortex and exerting protective function to neurons. Keywords: Recombinant Human Erythropoietin (r-HuEPO); Brain Injury; Survivin; NF-κB; Apoptosis 1. Introduction 2.2. Experimental Animal and Groups Survivin was one of the new-found genes