严重创伤对细胞免疫和树突状细胞抗原提呈功能影响.pdf

细胞的增殖指数（PI ）明显高于创伤组小鼠，此外，在抗原提呈过程中，1-MT 对脾DC 刺激脾非黏附细胞的增殖也有一定的影响。创伤小鼠脾DC 在抗原提呈 过程中分泌的 IL-12 比正常小鼠明显降低（P0.05 ），IL-10 则无明显差别；而 脾DC 与内毒素共培养过程中，创伤小鼠脾DC 分泌的IL-12 比正常小鼠明显升 高（P0.05 ），IL-10 则明显降低；创伤24 h 后，脾成熟DC 比正常组显著减少 （P0.05 ），未成熟DC 则显著增多（P0.05 ）；腹股沟淋巴结DC 总数量，成 熟DC 和未成熟DC 的数量比正常组均显著降低（P0.01 ）；肠系膜淋巴结DC 总数量，成熟DC 和未成熟DC 的数量也都比正常组显著下降（P0.01 ）。 结论： （1）建立了小鼠严重创伤模型，体内外的实验证明严重创伤会导致机体细胞免 疫功能下降 （2）细胞免疫功能的下降与DC 数量、成熟状态和表型及DC 抗原提呈和分泌细 胞因子功能的变化密切相关。 （3）针对创伤后的免疫失调，即创伤后抗原提呈功能下降而炎症因子活性增加， 并初步证明体内补充淋巴结细胞对促进创伤的恢复有一定的积极作用 关键词：创伤，细胞免疫，树突状细胞，细胞治疗 3 The Effect of trauma on cell immunity function and antigen presenting ability of Dendritic cells ABSTRACT Objective:Study DC’s changes after trauma and the relations between these changes and the disorderly cell immunity under trauma, and explore the mechanism of these changes following trauma. Learning the mechanism of DC’s involvement in trauma will undoubtedly spur new therapeutic strategy and drug development to protect human from severe trauma. Methods: With the model of trauma (hemorrhagic injury combined with closed fracture), DTH responses were induced by DNFB and FITC to evaluation of cell immunity function after trauma. In addition, the FITC+ + + , CD11c and MHC II cells in pooled inguinal lymph nodes were analysed by FACScan after FITC skin painting.In both early and later stage of DTH response, Cell transfer sensitization for DTH induced by DNFB after trauma in mice. DCs from spleens were separated by the method of Nycodenz density gradient enrichment. The low-density cells (3 %-5 % of the total) as DC were collected at the interface. Then their ability