大鼠超急性期和急性期脑梗死后eswan序列与水通道蛋白9表达的.doc

大鼠脑梗死后ESWAN序列与AQP4表达的相关性研究 ，程敬亮*，张勇郑州大学第一附属医院科 郑州 450052 [摘要] 目的 分析大鼠超急性期和急性期脑梗死时ESWAN序列的表现，探讨水通道蛋白4（AQP4）的表达规律。方法 选取健康雄性SD大鼠70只随机分为两组，实验组（56只）建立大脑中动脉栓塞（MCAO）模型，对照组（14只）不插入线栓，每组又分为术后1h、3h、6h、9h、12h、24h、72h组。对两组大鼠进行常规MRI序列T1WI、T2WI及DWI、ESWAN序列扫描。观察实验组大鼠梗死区和对照组相应区域不同时间点在ESWAN序列上的表现并测定SWAN信号值及相位弧度值；之后进行免疫组织化学染色分析梗死区AQP4的表达情况。结果 实验组大鼠MCAO后，梗死核心SWAN信号值在MCAO后1h时信号下降，1~12h时间段轻微波动，之后升高，于24h~72h有明显升高，72h时信号明显高于基线。不同时间点实验组梗死侧SWAN信号值与对照组相比，差异均具有统计学意义（P＜0.05）。相位弧度值在1h信号明显上升，1~24h逐步下降，24h时达最低水平，值低于基线，24h~72h又有所回升。不同时间点实验组梗死侧相位弧度值与对照组相比，差异均无统计学意义（P＞0.05）。在72h内，梗死区AQP4的表达逐步增加，与SWAN信号值呈正相关（r=0.85,P＜0.05）结论 超急性和急性脑梗死时ESWAN的信号改变可以间接反映AQP4的表达变化。 [关键词] 脑梗死；磁共振成像；ESWAN序列；水通道蛋白4；脑水肿6篇。手机E-mail：460620608@。 YANG Lu, CHENG Jingliang*, ZHANG Yong, BAI Jie,LIN Yanan (MR Division,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China) [Abstract] Objective To study the performance of ESWAN sequence after experimental cerebral infarction in rats and to explore the expression of Aquaporin 4 (AQP4) . Methods 70 male healthy SD rats were enrolled in the study. The rats were divided into two groups with the randomization principle.The experimental group of 56 rats established MCAO models. The control group of 14 rats do the same operation as the experimental group except insert the line tied. The rats of two group were divided into 1h,3h,6h,9h,12h,24h,72h group respectively.All rats performed axial T1-weighted imaging, T2-weighted imaging, diffusion-weighted imaging, ESWAN sequence.To Observe the performance of ESWAN sequence of the infarcted area of experimental rats and the corresponding region of the control group at different time points and measure the SWAN signal value and phase radians value. Then use the immunohistochemical staining to explore the AQP4 expression of infarcted area.Results The signal intensity of infarct core on SWAN decreased rapidly after 1h, minor fluctuated in 1~12h and then elevated. It showed significantly increased at 24h ~72