舒林酸硫化物对人乳腺癌细胞SKBR-3增殖与凋亡的影响.docVIP

舒林酸硫化物对人乳腺癌细胞SKBR-3增殖与凋亡的影响 周运江，王虎，随何欢，李丽，黄家君* （400016 重庆，重庆医科大学药学院药理学教研室，重庆市生物化学与分子药理学重点实验室） 【摘要】 目的：探讨舒林酸硫化物对人乳腺癌细胞SKBR-3增殖与凋亡的影响及其可能机制。方法：不同浓度的舒林酸硫化物（20 μmol/L，40 μmol/L，80 μmol/L）分别处理SKBR-3细胞，利用MTT法和流式细胞术来观察舒林酸硫化物对SKBR-3细胞增殖和凋亡的影响；Western blot检测细胞中Bcl-2、Bax、细胞色素C、Caspase-3、Cleaved caspase-3蛋白水平。结果：舒林酸硫化物抑制SKBR-3细胞的增殖，同时促进细胞的凋亡。舒林酸硫化物在作用SKBR-3细胞24 h后，与正常组比较Bcl-2和线粒体细胞色素C蛋白水平显著下降（P0.05），Bax、Cleaved caspase-3和胞质细胞色素C蛋白水平显著升高（P0.05）， 而Caspase-3蛋白水平差异没有显著性（P0.05）。结论：舒林酸硫化物能够抑制细胞的增殖，并且促进细胞的凋亡。其促进细胞凋亡的机制可能与下调Bcl-2蛋白的表达，上调Bax蛋白的表达，诱导细胞色素C从线粒体中释放，最终激活Caspase-3蛋白有关。 【关键词】：舒林酸硫化物；SKBR-3细胞；增殖；凋亡；Bcl-2 Effects of sulindac sulfide on proliferation and apoptosis of SKBR-3 cells Zhou Yunjiang, Wang Hu, Sui Hehuan, Li Li, Huang Jiajun* (Department of Pharmacology, Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016) *Corresponding Author: Huang Jiajun, E-mail: huangjiajun1964@163.com [Abstract] Objective: This study is to investigate the effects of sulindac sulfide on proliferation and apoptosis of SKBR-3 cells, and then explore its potential molecular mechanism. Methods: SKBR-3 cells were incubated with different concentrations of sulindac sulfide (20 μmol/L, 40 μmol/L, 80 μmol/L). Apoptosis and proliferation of SKBR-3 cells were analyzed by using flow cytometry and MTT assay, respectively. Western blot was used to test the protein expression of Bcl-2, Bax, cytochrome c, caspase-3 and cleaved caspase-3. Results: Sulindac sulfide inhibited the proliferation of SKBR-3 cells and promoted the apoptosis of cells. After 24 hours of incubation, the protein expression of Bcl-2 and mitochondrial cytochrome c were markedly reduced (P0.01), the protein expression of Bax, cytosolic cytochrome c and cleaved caspase-3 were significantly increased (P0.01), the protein expression of caspase-3 remained about the same (P0.05). Conclusion: Sulindac sulfide significantly inhibit the proliferation of SKBR-3 cells and promote cells a