antiproliferative activity of cinnamomum cassia constituents and effects of pifithrin-alpha on their apoptotic signaling pathways in hep g2 cells抗增殖活动肉桂的成分和影响pifithrin-alpha消息灵通的g2细胞凋亡信号通路.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 492148, 6 pages doi:10.1093/ecam/nep220 Original Article Antiproliferative Activity of Cinnamomum cassia Constituents and Effects of Pifithrin-Alpha on Their Apoptotic Signaling Pathways in Hep G2 Cells Lean-Teik Ng1 and Shu-Jing Wu2 1 Department of Agricultural Chemistry, National Taiwan University, No.1, Roosevelt Road Section 4, Taipei 106, Taiwan 2 Department of Nutritional Health, Chia-Nan University of Pharmacy and Science, 60, Erh-Jen Road, Section 1, Jen-Te, Tainan 717, Taiwan Correspondence should be addressed to Shu-Jing Wu, wsj268@.tw Received 25 August 2009; Accepted 25 November 2009 Copyright © 2011 L.-T. Ng and S.-J. Wu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Cinnamaldehyde (Cin), cinnamic acid (Ca) and cinnamyl alcohol (Cal), major constituents of Cinnamomum cassia, have been shown to possess antioxidant, anti-inflammatory, anticancer and other activities. In this study, our aim was to evaluate the antiproliferative activity of these compounds in human hepatoma Hep G2 cells and examine the effects of pifithrin-alpha (PFTα; a specific p53 inhibitor) on their apoptotic signaling transduction mechanism. The antiproliferative activity was measured by XTT assay. Expression of apoptosis-related proteins was detected by western blotting. Results showed that at a concentration of 30 μM, the order of antiproliferative activity in Hep G2 cells was Cin Ca Cal. Cin (IC50 9.76 ± 0.67 μM) demonstrated an antiproliferative potency as good as 5-fluorouraci