antiprotozoan and antiviral activities of non-cytotoxic truncated and variant analogues of mussel defensinantiprotozoan non-cytotoxic截断和抗病毒活动和贻贝defensin变体类似物.pdfVIP

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antiprotozoan and antiviral activities of non-cytotoxic truncated and variant analogues of mussel defensinantiprotozoan non-cytotoxic截断和抗病毒活动和贻贝defensin变体类似物.pdf

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antiprotozoan and antiviral activities of non-cytotoxic truncated and variant analogues of mussel defensinantiprotozoan non-cytotoxic截断和抗病毒活动和贻贝defensin变体类似物

Advance Access Publication 18 August 2004 eCAM 2004;1(2)167–174 doi:10.1093/ecam/neh033 Original Article Antiprotozoan and Antiviral Activities of Non-cytotoxic Truncated and Variant Analogues of Mussel Defensin 1 2 3 Philippe Roch , Alain Beschin and Eric Bernard 1Pathogènes et Immunité, UMR Ecosystèmes Lagunaires, Université de Montpellier 2, France, 2Department of Immunology, Parasitology and Ultrastructure, Flemish Interuniversity Institute for Biotechnology, Free University Brussels (VUB), Belgium and 3 Infectious Rétrovirales et Signalisation Cellulaire, UMR 5121, Institut de Biologie, Université de Montpellier 1, France We previously reported the crucial role displayed by loop 3 of defensin isolated from the Mediterranean mussel, Mytilus galloprovincialis , in antibacterial and antifungal activities. We now investigated antipro- tozoan and antiviral activities of some previously reported fragments B, D, E, P and Q. Two fragments (D and P) efficiently killed Trypanosoma brucei (ID50 4–12 M) and Leishmania major (ID50 12–45 M) in a time/dose-dependent manner. Killing of T. brucei started as early as 1 h after initiation of contact with fragment D and reached 55% mortality after 6 h. Killing was temperature dependent and a temperature of 4C efficiently impaired the ability to kill T. brucei. Fragments bound to the entire external epithe- lium of T. brucei. Prevention of HIV-1 infestation was obtained only with fragments P and