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Advances in Bioscience and Biotechnology, 2012, 3, 238-248 ABB /10.4236/abb.2012.33033 Published Online June 2012 (http://www.SciRP.org/journal/abb/) Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density Pichia pastoris bioreactor cultivations 1 2 3 1 Magnus Sjöblom , Linda Lindberg , Jan Holgersson , Ulrika Rova 1Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, Luleå, Sweden 2Absorber AB, Stockholm, Sweden 3Department of Clinical Chemistry Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Email: ulrika.rova@ltu.se Received 15 February 2012; revised 26 March 2012; accepted 5 April 2012 ABSTRACT 1. INTRODUCTION The methanol inducible alcohol oxidase 1 promoter The methylotrophic yeast Pichia pastoris is a robust mi- croorganism for industrial cultivation and has been used and the Saccharomyces cerevisiae alpha-factor prepro to express and secrete several recombinant proteins in secretion signal were used to drive expression and the g per litre range [1]. However, the secretion levels secretion of a mucin-type fusion protein by Pichia with this yeast are still highly variable and in several pastoris in 1 L scale bioreactors. The aim of the study