白藜芦醇对衰老昆明小鼠肝细胞作用机制探析.docVIP

白藜芦醇对衰老昆明小鼠肝细胞作用机制探析[摘要] 目的:观察白藜芦醇对衰老雌性昆明小鼠肝细胞凋亡相关蛋白Bcl-2、Bax及其抗氧化能力的影响,研究白藜芦醇延缓肝细胞衰老的作用和机制。方法:采用自然衰老雌性昆明小鼠,给予不同剂量的白藜芦醇,用免疫组化方法观察肝细胞凋亡相关蛋白Bcl-2、Bax的表达,用生化法测定血清总超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果:衰老对照组与青年对照组比较,Bcl-2表达明显降低,Bax表达升高,血清中SOD活力下降、MDA含量显著升高(P 1.5 肝组织中Bcl-2及Bax的测定 用免疫组织化学SABC法,Bcl-2定位于核膜、粗面内质网和线粒体膜上,Bax定位于胞质。免疫细胞化学结果判定采用组织化学评分法。阳性染色为胞质内出现棕黄色颗粒,以i代表染色深浅,根据细胞着色的强弱分为4个等级:无着色0分,淡黄色1分,棕黄色2分,棕褐色3分。该评分法可反映单个细胞是否具有阳性表达及表达强弱,更客观地评价细胞蛋白的表达情况。 1.6 统计学分析 数据以均数±标准差(x±s)表示,采用SPSS 11.0软件分析,实验结果采用t检验,P [3]Shiraishi N, Sato K, Yasuda K, et al. Multivariate prognostic study on large gastric cancer [J]. J Surg Oncol,2007,96(1):14-18. [4]Liu HS. Studies on effects of resveratrol in inhibition growth of C6 and TJ905 glioma cell [J]. Chin Tradit Herb Drugs,2005,36(11):1695-1696. [5]Aggarwal S, Ichikawa H, Takada Y, et al. Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of I kappa B alpha kinase and Akt activation [J]. Mol Pharmacol,2006,69(1):195-206. [6]Sheng L, Anl F. Research on resveratrol’s effects of suppressing growth and inducing apoptosis of GBC cells [J]. J Chin Med Mater,2005,28(6):489-491. [7]Fulda S, Debatin KM. Resveratrol mediated sensitisation to TRA IL induced apoptosis depends on death receptor and mitochondrial signaling [J]. Eur J Cancer,2005,41(5):786-798. [8]Atten MJ, Godoy RE, Attar BM, et al. Resveratrol regulates cellular PKC alpha and delta to inhibit growth and induce apoptosis in gastric cancer cells [J]. Invest New Drug,2005,23(2):111-119. [9]Markovits J, Wang Z, Carr B, et al. Differential effect of two growth inhibitory K vitamin analogs on cell cycle regulating proteins in human hepatoma cells [J]. Life Sci,2003,72(24):2769-2784. [10]Oztopcu P, Kabadere S, Mercangoz A, et al. Comparison of vitamin K effecting growth of rat glioma and human glioblastoma multiforme cells in vitro [J]. Acta Neurol Belg,2004,104(