转NTHK1基因对杨树各器官中Na+、K+分布影响.docVIP

转NTHK1基因对杨树各器官中Na+、K+分布影响摘要：以盐渍生境下1～2年生107和18—1杨树为材料，对不同树龄和地径的杨树各营养器官中Na+、K+分布变化进行了研究。结果表明，Na+、K+在杨树各营养器官中分布不同且差异显著。不同树龄和地径的107杨树的根、茎、叶部位的Na+、K+含量变化都很明显，含NTHK1的转基因18—1杨树根部的Na+、K+含量基本保持恒定，茎、叶部位的Na+含量变化均不明显，茎部的K+含量随着树龄的增加而升高，叶片部位的K+含量随着树龄的增加而降低。研究表明，在盐渍生境中种植的含NTHK1的转基因18—1杨树比107杨树更加耐盐，可以更好地抵御盐胁迫，维持离子平衡，NTHK1基因可能是通过增强转基因杨树聚集有害Na+至液泡的能力，以避免细胞质中过高的Na+对细胞造成伤害，从而提高了转基因植株的耐盐性。 关键词：杨树；盐胁迫；NTHK1基因；树龄；地径；器官；Na+；K+ 中图分类号：S792.11 文献标识码：A DOI编码：10.3969/j.issn.1006—6500.2012.05.001 Effect of Transgene NTHK1 on Distribution of Na+ and K+ in Different Organs of Poplar SONG Jian1， ZHOU Xiang—ming1， HAO Zhi—yu1， WANG Shu1， WANG Peng2， LIU Zhong—qi1 （1.Tianjin Research Center of Agricultural Biotechnology，Tianjin 300192，China； 2. Life Science College，Tianjin Normal University，Tianjin 300387，China） Abstract： Researched the distribution of Na+ and K+ in different vegetative organs of poplar material 107’ and ‘18—1’， which were 1～2 year old tree and different in ground diameter in saline environment. The results indicated that the distribution of Na+ and K+ in poplar vegetative organs was different.The change of content of Na+ and K+ in roots， stems and leaves of 107’ was obvious with different tree age and different ground diameter ，the content of Na+ and K+ in roots of 18—1’ containing NTHK1 was fundamentally equal with different tree age and ground diameter. The content of K+ in stems raised with increase of tree age，the content of K+ in leaves lowered with increase of tree age，the content of Na+ in stems and leaves was lesser .The features that poplar ‘18—1’ was more applicable the saline than ‘107’，which can better resist to salt stress and maintain ion homeostasis. The effect of NTHK1 may be limitted a large number of Na+ in the vacuole and reduced ion toxicity of high consistency Na+ to cell， so the salt tolerance ability of 18—1 containing NTHK1 was enhanced. Key words： poplar； salt stress； NTHK1 gene；tree age；ground diameter；vegetative organs； Na+； K+ 土壤盐渍化是影响农业生产