首乌方对左旋多巴诱发异动症模型大鼠行为学及脑-中国试验动物学报.doc

首乌方对左旋多巴诱发异动症模型大鼠脑内氨基酸类神经递质水平的影响( 刘 洋，焦 玥，孙丹丹，王丹巧*，赵小亮，李涛，张美玉 （北京市中医药防治重大疾病基础研究重点实验室 中国中医科学院医学实验中心, 北京 100700） 中图分类号：R 277.7; R285.5; R742.5; R971+.5 【摘要目的 诱发异动症模型大鼠行为学及。方法 采用6-羟多巴胺（6-OHDA）脑内立体定位注射造成偏侧帕金森病（PD）大鼠模型，成模动物另设假手术组。所有动物连续给药22 d，d 22采用微透析技术对大鼠纹状体细胞外液进行取样，并γ氨基丁酸（GABA）水平动态变化。结果P0.05, P0.01）。结论 大鼠学症状，其作用机制或与其有关。 γ-氨基丁酸 Effect of SWF on amino acids neurotransmitters in levodopa-induced dyskinesia model rats LIU Yang, JIAO Yue, SUN Dan-dan, WANG Dan-qiao*, ZHAO Xiao-liang, LI Tao, ZHANG Mei-yu, (Beijing Key Laboratory of TCM Basic Research on Prevention and Treatment of Major Disease, Experimental Research Center, China Academy of Chinese Medical Science, Beijing 100700, China) 【Abstract】 Objective To investigate, in terms of behaviors and striatal levels of amino acids neurotransmitters, the effect of Shouwu Recipe (SWF), a traditional Chinese medicine compound, on levodopa-induced dyskinesia (LID) model rats, and further to explore its mechanism. Methods SD rats were injected with 6-hydroxydopamine (6-OHDA) into the right substantia nigra pars compacta and the ventral tegmental area to produce hemi-lateral nitro-striatum lesion. 6-OHDA lesioned rats were daily given L-DOPA (30 mg of Madopar/kg/d, equivalent to 24 mg of L-DOPA and 6 mg of benserazide, i.g.), or L-DOPA plus SWF (containing 18, or 36 g/kg/d of crude medicinals, i.g.). All rats were treated for 22 days, once per day. During the treatment, abnormal involuntary movements (AIMs) were evaluated. On 22nd day, in vivo microdialysis was used to collect striatal extracellular fluid as the rats were in sober and free-moving condition. All dialysates were analyzed by high performance liquid-fluorescence (HPLC-FLD) detection. Results After 21-day’s treatment, AIM scores of L-DOPA-treated rats were higher than the sham-operated rats (P0.01). L-DOPA plus high dose of SWF-treated rats had lower AIM scores than L-DOPA-treated rats (P0.05). On 22nd day, in L-DOPA-treated rats, striatal levels of glutamate