bone marrow transplantation results in human donor blood cells acquiring and displaying mouse recipient class i mhc and cd45 antigens on their surface骨髓移植的结果在人类供体血液细胞获取和显示鼠标受体类mhc和cd45的表面抗原.pdfVIP

Bone Marrow Transplantation Results in Human Donor Blood Cells Acquiring and Displaying Mouse Recipient Class I MHC and CD45 Antigens on Their Surface 1 1 1 1,2 1,3 Nobuko Yamanaka , Christine J. Wong , Marina Gertsenstein , Robert F. Casper , Andras Nagy , Ian M. Rogers1,2* 1 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, 2 Department of Obstetrics and Gynecology, University of Toronto, Toronto, Canada, 3 Department of Molecular Genetics, University of Toronto, Toronto, Canada Abstract Background: Mouse models of human disease are invaluable for determining the differentiation ability and functional capacity of stem cells. The best example is bone marrow transplants for studies of hematopoietic stem cells. For organ studies, the interpretation of the data can be difficult as transdifferentiation, cell fusion or surface antigen transfer (trogocytosis) can be misinterpreted as differentiation. These events have not been investigated in hematopoietic stem cell transplant models. Methodology/Principal Findings: In this study we investigated fusion and trogocytosis involving blood cells during bone marrow transplantation using a xenograft model. We report that using a standard SCID repopulating assay almost 100% of the human donor cells appear as hybrid blood cells containing both mouse and human surface antigens. Conclusion/Significance: Hybrid cells are not the result of cell-cell fusion events but appear to be due to efficient surface antigen transfer, a process referred to as trogocytosis. Antigen transfer appears to be non-random and includes all donor cells regar