基于斑马鱼行为变化的苯酚监测研究.pdfVIP

安徽农业科学，Journal of Anhui Agri. Sci. 2017 ,45( 1) :83 -邸，93 基于斑马鱼行为变化的苯盼监测研究 1 周绍辉1 ，杜娟娟1 ，孙中也11 ，李梅1* ，连南南2 ，宋武昌2 (1.山东建筑大学市政与环境工程学院，山东济南 250101 ;2. 山东省城市供排水水质监测中心，山东济南 25∞21) 摘要 [目的]探究一种可行的水体苯盼监测预警技术。[方法]以斑马鱼作为模式生物，采用半静态试验法，考察了苯盼对斑马鱼的急 性毒性及行为强度影响。[结果]苯盼对斑马鱼毒性为中毒物质;与对照纽相比，苯盼试验纽斑马鱼行为强度变化板显著，苯盼对斑马鱼 的毒性呈明显的时间-效应和剂量-效应关系。苯盼胁迫下，斑马鱼行为变化符合环珑压力模型。苯盼浓度越高，斑马鱼行为强度变 化越显著。[结论]基于斑马鱼行为变化的生物在线监测技术是实现水体苯盼预警的有效手段。 关键词 斑马鱼;苯盼;急性毒性;行为变化 中图分类号 5912 文献标识码 A 文章编号 0517 -6611(2017)01-∞83 -03 Research of Phenol Moniωdng Based on the Behavior Changes of Brachydanio re.而 ZHOU Shao-hui , DU Juan-juan , SUN Zho昭-xun ， LI Meγet aI (School of Municipal and Environmental Engineeri吨， 5handong Jianzhu University , Jinan , 5handong 250101) Abstr回t [0均ective] To explore a fe由ible technology for phenol early warning in water body. [Metbod] Acute toxicity and behavioral chan- ges of Brachydanio rerio (臼 a model organism) were studied under phenol stress using semi-static test metbods. [Result] The results showed that phenol is mildly toxic to Brachydαnio rerio; compared witb tbe control group , behavioral changes of Brα， chydanio rerio in tbe phenol test group were extremely significant. Phenol toxicity tests on Brachydt阴阳时rio showed an obvious time-effect and dose-effect relationship. Under phenol stress , behavior changes of Brachydanio rerio fit wi也 tbe environmental stress mode l. 8ehavior changes of Brachydanio rerio were more significant under higher concentration of phenol. [Conclusion] Online monitoring technology based on tbe behavior changes of Brachydanio re- rio is an effective way to realize phenol early warning in water body. Key words Brachydanio rerio; Phenol; Acute toxicity; 8ehavior changes 苯酣是-类中等毒性的有机化学污染物，进入生物体后 (斑马鱼〉急性毒性测定方法》配置要求配制，pH 为 7.8 士 会发生一些列生物反应[1] ，并通过生物链积