comparative analysis of fecal microbiota in infants with and without eczema比较分析的粪便微生物群在婴儿和湿疹.pdfVIP

Comparative Analysis of Fecal Microbiota in Infants with and without Eczema 1,2 2 2 2 2 2 Pei-Ying Hong , Bee Wah Lee , Marion Aw , Lynette Pei Chi Shek , Gaik Chin Yap , Kaw Yan Chua , Wen-Tso Liu1,3* 1 Division of Environmental Science and Engineering, National University of Singapore, Singapore, Singapore, 2 Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore, 3 Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America Abstract Eczema is a chronic form of childhood disorder that is gaining in prevalence in affluent societies. Previous studies hypothesized that the development of eczema is correlated with changes in microbial profile and composition of early life endemic microbiota, but contradictory conclusions were obtained, possibly due to the lack of minimization of apparent non-health related confounders (e.g., age, antibiotic consumption, diet and mode of delivery). In this study, we recruited seven caesarean-delivered and total formula-fed infants, and comparatively examined the early-life endemic microbiota in these infants with and without eczema. Using 16S pyrosequencing, infants’ fecal microbiota were observed to comprise Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes as the four main phyla, and the presence and absence of specific populations within these four phyla are primarily mediated by ageing. Quantitative analysis of bacterial targets on a larger sample size (n = 36 at 1, 3, and 12 months of age) revealed that the abundances of Bifidoba