development and fibronectin signaling requirements of the zebrafish interrenal vessel发展和纤连蛋白信号要求的斑马鱼interrenal船.pdfVIP

Development and Fibronectin Signaling Requirements of the Zebrafish Interrenal Vessel 1 1 2,3 1 Chih-Hao Chiu , Chih-Wei Chou , Shinji Takada , Yi-Wen Liu * 1 Department of Life Science, Tunghai University, Taichung, Taiwan R.O.C, 2 Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi, Japan, 3 Department of Basic Biology, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan Abstract Background: The early morphogenetic steps of zebrafish interrenal tissue, the teleostean counterpart of the mammalian adrenal gland, are modulated by the peri-interrenal angioblasts and blood vessels. While an organized distribution of intra- adrenal vessels and extracellular matrix is essential for the fetal adrenal cortex remodeling, whether and how an intra- interrenal buildup of vasculature and extracellular matrix forms and functions during interrenal organogenesis in teleosts remains unclear. Methodology and Principal Findings: We characterized the process of interrenal gland vascularization by identifying the interrenal vessel (IRV); which develops from the axial artery through angiogenesis and is associated with highly enriched Fibronectin (Fn) accumulation at its microenvironment. The loss of Fn1 by either antisense morpholino (MO) knockdown or genetic mutation inhibited endothelial invasion and migration of the steroidogenic tissue. The accumulation of peri-IRV Fn requires Integrin a5 (Itga5), with its knockdown leading to interrenal and IRV morphologies phenocopying those in the fn1 morphant and mutant. fn1b, another known fn gene in zebrafish, is however not involved in the IRV formation. The distribution pattern of peri-IRV Fn could be modulated by the blood flow,