development and evaluation of two simple, rapid immunochromatographic tests for the detection of yersinia pestis antibodies in humans and reservoirs开发和评估两个简单、快速检测试纸检测鼠疫杆菌抗体的检测在人类和水库.pdfVIP

Development and Evaluation of Two Simple, Rapid Immunochromatographic Tests for the Detection of Yersinia pestis Antibodies in Humans and Reservoirs 1 2 1 3 4 Minoarisoa Rajerison , Sylvie Dartevelle , Lalao A. Ralafiarisoa , Idir Bitam , Dinh Thi Ngoc Tuyet , 1 2 1 Voahangy Andrianaivoarimanana , Faridabano Nato , Lila Rahalison * ´ ´ ´ 1 Unite Peste - Institut Pasteur de Madagascar, Antananarivo, Madagascar, 2 Departement de Biologie Structurale et Chimie – Laboratoire de Production de Proteines ´ ´ Recombinantes et d’Anticorps - Institut Pasteur de Paris, Paris, France, 3 Laboratoire d’Entomologie Medicale – Service de Parasitologie - Institut Pasteur d’Algerie, ´ Hamma-Alger, Algeria, 4 Departement de Microbiologie - Institut Pasteur de Nha-Trang, Nha-Trang, Viet Nam Abstract Background: Tools for plague diagnosis and surveillance are not always available and affordable in most of the countries affected by the disease. Yersinia pestis isolation for confirmation is time-consuming and difficult to perform under field conditions. Serologic tests like ELISA require specific equipments not always available in developing countries. In addition to the existing rapid test for antigen detection, a rapid serodiagnostic assay may be useful for plague control. Methods/Prin