developmental regulation and spatiotemporal redistribution of the sumoylation machinery in the rat central nervous system发育调控和时空分配sumoylation机械的大鼠中枢神经系统.pdfVIP

Developmental Regulation and Spatiotemporal Redistribution of the Sumoylation Machinery in the Rat Central Nervous System ´ 1,2 ´ 2 ´ 1,2 1,2 ´ 1,2 Celine Loriol , Josephine Parisot , Gwenola Poupon , Carole Gwizdek , Stephane Martin * ´ 1 Institut de Pharmacologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, Valbonne, France, 2 University of Nice - Sophia-Antipolis, Nice, France Abstract Background: Small Ubiquitin-like MOdifier protein (SUMO) is a key regulator of nuclear functions but little is known regarding the role of the post-translational modification sumoylation outside of the nucleus, particularly in the Central Nervous System (CNS). Methodology/Principal Findings: Here, we report that the expression levels of SUMO-modified substrates as well as the components of the sumoylation machinery are temporally and spatially regulated in the developing rat brain. Interestingly, while the overall sumoylation is decreasing during brain development, there are progressively more SUMO substrates localized at synapses. This increase is correlated with a differential redistribution of the sumoylation machinery into dendritic spines during neuronal maturation. Conclusions/Significance: Overall, our data clearly demonstrate that the sumoylation process is developmentally regulated in the brain with high levels of nuclear sumoylation early in the development suggesting a role for this post-translational modification during the synaptogenesis period and a redistribution of the SUMO system towards dendritic spines at a later developmental stage to modulate synaptic