dige proteome analysis reveals suitability of ischemic cardiac in vitro model for studying cellular response to acute ischemia and regeneration消化蛋白质组分析显示缺血性心脏体外模型的适用性研究细胞反应的急性缺血和再生.pdfVIP

DIGE Proteome Analysis Reveals Suitability of Ischemic Cardiac In Vitro Model for Studying Cellular Response to Acute Ischemia and Regeneration 1 1 2 2,3 4,5 Sina Haas , Heinz-Georg Jahnke , Nora Moerbt , Martin von Bergen , Seyedhossein Aharinejad , 5,6 1 Olena Andrukhova , Andrea A. Robitzki * ¨ 1 Division of Molecular Biological-Biochemical Processing Technology, Center for Biotechnology and Biomedicine, Universitat Leipzig, Leipzig, Germany, 2 Department of Proteomics, Helmholtz Centre for Environmental Research, Leipzig, Germany, 3 Department of Metabolomics, Helmholtz Centre for Environmental Research, Leipzig, Germany, 4 Department of Cardiac Surgery, Center of Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria, 5 Department for Cardiovascular Research, Center of Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria, 6 Department for Biomedical Sciences, Institute of Pathophysiology, University of Veterinary Medicine, Vienna, Austria Abstract Proteomic analysis of myocardial tissue from patient population is suited to yield insights into cellular and molecular mechanisms taking place in cardiovascular diseases. However, it has been limited by small sized biopsies and complicated by high variances between patients. Therefore, there is a high demand for suitable model systems with the capability to simulate ischemic and cardiotoxic effects in vitro, under defined conditions. In this context, we