effect of hydrogen peroxide and superoxide anions on cytosolic ca2+ comparison of endothelial cells from large-sized and small-sized arteries过氧化氢和超氧化物阴离子对内皮细胞的胞质钙离子比较大型和小型的动脉.pdfVIP

Effect of Hydrogen Peroxide and Superoxide Anions on Cytosolic Ca2+: Comparison of Endothelial Cells from Large-Sized and Small-Sized Arteries Lei Sun, Ho-Yan Yau, On-Chai Lau, Yu Huang, Xiaoqiang Yao* School of Biomedical Sciences and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, China Abstract We compared the Ca2+ responses to reactive oxygen species (ROS) between mouse endothelial cells derived from large- sized arteries, aortas (aortic ECs), and small-sized arteries, mesenteric arteries (MAECs). Application of hydrogen peroxide (H O ) caused an increase in cytosolic Ca2+ levels ([Ca2+] ) in both cell types. The [Ca2+] rises diminished in the presence of 2 2 i i U73122, a phospholipase C inhibitor, or Xestospongin C (XeC), an inhibitor for inositol-1,4,5-trisphosphate (IP ) receptors. 3 Removal of Ca2+ from the bath also decreased the [Ca2+] rises in response to H O . In addition, treatment of endothelial i 2 2 cells with H O reduced the [Ca2+] responses to subsequent challenge of ATP. The decreased [Ca2+] responses to ATP were 2 2 i i resulted from a pre-depletion of intracellular Ca2+ stores by H O . Interestingly, we also found that Ca2+ store depletion was 2 2 more sensitive to H O