schwann cells overexpressing fgf-2 alone or combined with manual stimulation do not promote functional recovery after facial nerve injury雪旺细胞overexpressing fgf-2单独或结合手动刺激不促进面神经损伤后功能恢复.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2009, Article ID 408794, 11 pages doi:10.1155/2009/408794 Research Article Schwann Cells Overexpressing FGF-2 Alone or Combined with Manual Stimulation Do Not Promote Functional Recovery after Facial Nerve Injury Kirsten Haastert,1 Maria Grosheva,2 Srebrina K. Angelova,2 Orlando Guntinas-Lichius,3 Emmanouil Skouras,4 Joern Michael,5 Claudia Grothe,1 Sarah A. Dunlop,6 and Doychin N. Angelov7 1 Institute of Neuroanatomy, Hannover Medical School and Center for Systems Neuroscience (ZSN), 30625 Hannover, Germany 2 Department of Otorhinolaryngology, University of Cologne, 50931 Cologne, Germany 3 Department of Otorhinolaryngology, Friedrich-Schiller University Jena, 07740 Jena, Germany 4 Department of Trauma, Hand and Reconstructive Surgery, University of Cologne, 50931, Germany 5 Department of Orthopaedic Surgery, University of Cologne, 50931 Cologne, Germany 6 School of Animal Biology, Western Australian Institute for Medical Research, The University of Western Australia, Crawley, Perth, WA 6009, Australia 7 Department of Anatomy I, University of Cologne, 50931, Germany Correspondence should be addressed to Doychin N. Angelov, angelov.anatomie@uni-koeln.de Received 25 February 2009; Accepted 8 July 2009 Recommended by George Perry Purpose. To determine whether transplantation of Schwann cells (SCs) overexpressing different isoforms of fibroblast growth factor 2 (FGF-2) combined with manual stimulation (MS) of vibrissal muscles improves recovery after facial nerve transection in adult ¨ rat. Procedures. Transected facial nerves were entubulated with collagen alone or