selection of ovine oocytes by brilliant cresyl blue staining选择绵羊的卵母细胞,灿烂甲酚蓝染色.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2012, Article ID 161372, 7 pages doi:10.1155/2012/161372 Research Article Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining Liqin Wang, Jiapeng Lin, Juncheng Huang, Jing Wang, Yuncheng Zhao, and Tong Chen Key Laboratory of Animal Biotechnology of Xinjiang and Key Laboratory of Grass Livestock Reproduction and Breed Biotechnology, Ministry of Agriculture, Animal Science Academy of Xinjiang Uygur Autonomous Region, Urumqi 830000, China Correspondence should be addressed to Juncheng Huang, h jc@ Received 24 January 2012; Revised 28 February 2012; Accepted 22 March 2012 Academic Editor: Brad Upham Copyright © 2012 Liqin Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers of cumulus cells and cytoplasm or cytoplasm uniformity and colors. This requires considerable experience to better identify oocyte quality because of the intense subjectivity involved (Gordon (2003), Madison et al. (1992) and De Loos et al. (1992)). BCB staining is a function of glucose-6-phosphate dehydrogenase (G6PD) activity, an enzyme synthesized in developing oocytes, which decreases in activity with maturation. Therefore, unstained oocytes (BCB−) ar