the comparative protective effects of ganoderma spores lipid and fish oil on n-methyl-n-nitrosourea-induced photoreceptor cell lesion in rats灵芝孢子比较保护作用的脂质和鱼油n-methyl-n-nitrosourea-induced感光细胞损伤的老鼠.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 903261, 10 pages doi:10.1155/2011/903261 Research Article The Comparative Protective Effects of Ganoderma Spores Lipid and Fish Oil on N-Methyl-N-Nitrosourea-Induced Photoreceptor Cell Lesion in Rats Yang Gao,1 Xin-Guo Deng,1 Na Li,1 Guang-Wei Luo,1 and Peter C. K. Chung2 1 State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China 2 Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China Correspondence should be addressed to Xin-Guo Deng, dengxg61@163.net Received 4 January 2011; Revised 4 March 2011; Accepted 7 March 2011 Copyright © 2011 Yang Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuc