the effect of ppar agonists on the migration of mature and immature eosinophilsppar激动剂的影响迁移的成熟和不成熟的嗜酸性粒细胞.pdfVIP

Hindawi Publishing Corporation PPAR Research Volume 2012, Article ID 235231, 8 pages doi:10.1155/2012/235231 Research Article The Effect of PPAR Agonists on the Migration of Mature and Immature Eosinophils Steven G. Smith,1 Haruki Imaoka,2 Neha Punia,1 Anam Irshad,1 Luke L. Janssen,1 Roma Sehmi,1 and Gail M. Gauvreau1 1 Department of Medicine, McMaster University, Hamilton, ON, Canada L8S 4K1 2 Department of Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan Correspondence should be addressed to Gail M. Gauvreau, gauvreau@mcmaster.ca Received 29 February 2012; Accepted 7 May 2012 Academic Editor: Raju Reddy Copyright © 2012 Steven G. Smith et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. PPARγ agonists can either enhance or inhibit eosinophil migration, which is a sum of directional migration (chemotaxis) and random cell movement (chemokinesis). To date, the effects of PPAR agonists on chemokinesis have not been examined. This study investigates the effects of PPARα, δ, and γ agonists on eosinophil migration and chemokinesis. Eosinophils purified from blood of atopic donors were preincubated with rosiglitazone (PPARγ agonist), GW9578 (PPARα agonist), GW501516 (PPARδ agonist), or diluent. The effects of PPAR agonists were examined on eosinophil chemokinesis, eotaxin-induced migration of eosinophils, and migration of IL-5Rα+ CD34+ cells. Expressions of CCR3, phospho-p38, phospho-ERK, and calcium release were also measured in eosinophils after rosiglitazone treatment. Low concentrations of rosiglitazone, but not GW9578 or GW501516,