the osteoprotective effect of herba epimedii (hep) extract in vivo and in vitro草的osteoprotective效果epimedii(玫瑰)提取体内和体外.pdfVIP

Advance Access Publication 26 July 2005 eCAM 2005;2(3)353–361 doi:10.1093/ecam/neh101 Original Article The osteoprotective effect of Herba epimedii (HEP) extract in vivo and in vitro 1,3 1 3,4 2,3 3,4 Fang Xie , Chun-Fu Wu , Wan-Ping Lai , Xu-juan Yang , Pik-Yuan Cheung , Xin-Sheng Yao2,*, Ping-Chung Leung5 and Man-Sau Wong3,4 1Department of Pharmacology and 2Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, 3State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, 4Open Laboratory of Chirotechnology of the Institute of Molecular, Technology for Drug Discovery and Synthesis, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR and 5Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, China Herba epimedii (HEP) is one of the most frequently used herbs prescribed for treatment of osteo- porosis in China. In the present study, the in vivo effects of HEP extract on bone metabolism were evaluated using 4-month-old ovariectomized (OVX) or sham-operated (Sham) female Sprague- Dawley rats orally administered with HEP extract (110 mg kg1d1), 17ß-estrogen (2 mg kg1d1) or its vehicle for 3 months. HEP extract significantly decreased urinary calcium excretion, suppressed serum alkaline phosphatase (ALP) activity