外源生长素和细胞分裂素对莴苣幼苗侧根原基发生-南京农业大学学报.PDF

南京农业大学学报 　2000 , 23 ( 1) : 19～22 　 Journal of Nanjing Agricultural University 外源生长素和细胞分裂素对莴苣幼苗侧根 原基发生和内源激素含量的影响 江 　玲 　周 　燮 (南京农业大学农学系 , 南京 210095) ( ) ( ) ( ) 摘要 　以莴苣幼苗为材料, 以适宜浓度的生长素类物质萘乙酸 NAA 、吲哚丁酸 IBA 和吲哚乙酸 IAA 处理根部 20～30 h , 可使大量的侧根原基得到启动和突起 , 10000 mg/ L 的NAA 、IBA 或 IAA 处理的侧根原基数分别为对照的 279 , ( ) ( ) 265 和 136 倍 ; 而用 6苄基腺嘌呤 6BA 和调吡脲 KT30 等细胞分裂素类物质及配合生长素类物质处理 , 则抑制 幼苗自发生根和生长素的促根效应。5000 mg/ L 的 6BA 几乎完全抑制了这些生长素的效应 , 同浓度的 KT30 对 NAA 、 IBA 和 IAA 的抑制率分别为738 % , 670 %和 677 % , 这与内源生长素含量的升高和细胞分裂素含量的下降直接相关。 关键词 　莴苣 ; 侧根原基 ; 生长素 ; 细胞分裂素 分类号 　S636201 The effect of auxins and cytokinins on the formation of lateral root primordia and the contents of endogenous hormones in lettuce seedlings Jiang Ling and Zhou Xie (Dept of Agronomy , Nanjing Agric Univ , Nanjing 210095) ABSTRACT 　Lettuce (L actuca s ativa L. ) was used as material and the results showed that , the initiation and formation of lateral root primordia (LRP) was significantly promoted 20 30 h after being treated with auxins such as NAA , IBA , IAA etc at a proper concentration , the number of LRP by treatment with 10000 mg/ L NAA , IBA or IAA were 279 , 265 or 136 times those of the control respectively. While CTKs such as 6BA and KT30 effectively inhibited the spontaneous and auxinsstimulated formation of LRP , the inhibition effect of 5. 000 mg/ L KT30 on the NAA , IBA of IAA were 738 % , 670 % or 677