系膜增生性肾小球肾炎患者血清Th1Th2细胞因子表达的变化.docVIP

系膜增生性肾小球肾炎患者血清Th1/Th2细胞因子表达的变化 程 悦,张静波,李芙蓉,王代红,袁发焕 (第三军医大学新桥医院肾内科全军肾脏病中心重庆市肾脏病研究所重庆 400037)γ、IL-4、IL-10）表达变化。方法 采用酶联免疫吸附实验（ELISA）测定10名正常人和25名慢性肾小球疾病患者（轻中度系膜增生性非IgAγ、IL-4、IL-10细胞因子水平。结果 系膜增生性肾小球肾炎患者血清INF-γ分泌水平较正常人明显增多（P0.0）（P0.0）（P0.05）γ/IL-4、INF-γ/IL-10值均显著高于正常人（P0.0）可能。γ；IL-4；IL-10 中图法分类号： 文献标识码：A Expression of Th1/Th2 cytokines in patients with Mesangial proliferative nephritis CHENG Yue,ZHANG Jing-bo,LI Fu-rong,Wang Dai-hong,YUAN Fa-huan(Department of Nephrology,Xinqiao Hospital,the Third Militeray Medical University,Chongqing 400037,China) ABSTRACT Objective: To investigate the expression of Th1 and Th2 cytokines including INF-γ,IL-4 and IL-10 in patients with Mesangial proliferative nephritis Methods: 25 patients with MsPGN and 10 healthy donors were enrolled in this study, and ELISA was adopted to determine the serum values of INF-γ,IL-4 and IL-10. Results: Serum values of IFN-γ were higher in MsPGN patients than that in healthy donors(P0.01), and IL-10 values in MsPGN patients were lower than that in healthy donors(p0.05). There was no significant differences in IL-4 values between MsPGN patients and healthy donors(P0.05).The INF-γ/IL-4 ratio and INF-γ/IL-10 ratio in MsPGN patients were significantly higher than those in healthy donors. (P0.01) Conclusion: The polarization of Th1 cytokine in MsPGN patients demonstrated that Th1 skewing may be involved in the pathogenesis of MsPGN. Key words：MsPGN; INF-γ;IL-4;IL-10 细胞因子在调节免疫细胞应答中起关键作用，Mosmann [1]将其分为Th1和Th2型细胞因子。Th1型细胞因子包括INF-γ、INF-α、INF-β和IL-2等，主要参与细胞免疫，介导细胞毒T细胞和巨噬细胞的活化以及迟发性变态反应；Th2型细胞因子包括IL-4、IL-5、IL-6和IL-10等，主要介导体液免疫，辅助B细胞分化和抗体形成。大量研究发现Th1和Th2型细胞因子失衡与多种免疫性疾病的发生发展密切相关。慢性肾小球肾炎的主要病理机制是免疫紊乱造成的肾脏炎症损伤，已有研究报道了慢性肾小球肾炎存在Th1/Th2细胞因子失衡，但结果存在较大分歧。由于不同的免疫应答通过分泌不同类型的细胞因子调节细胞免疫和体液免疫，影响疾病的发生、类型和预后，我们推测不同的病理类型可能影响患者Th1/Th2失衡状态。 系膜增生性肾小球肾炎是最为常见的慢性肾小球肾炎病理类型，其免疫发生机制尚不清楚。本实验拟从临床角度，以单一的系膜增生性肾小球肾炎为观察对象，观察患者外周血细胞因子水平是否存在Th1/Th2失衡，以期进一步探讨系膜增生性肾小球肾炎的免疫发病机制，为寻找新的治疗方法奠定基础。 1 对象与方法 1.1 研究对象与试剂 10例正常人和25例系膜增生性肾小球肾炎患者（为