宿主自身卵巢与移植胚鼠卵巢生长发育相互影响的实验研究.docVIP

宿主自身卵巢与移植胚鼠卵巢生长发育 相互影响的实验研究 秦海燕 黄学惠1 马芳1（1昆明医学院, 地址:昆明市西昌路295号昆明医学院第一附属医院妇科，邮政编码：650032） 【摘要】：目的 探讨宿主自身卵巢与移植胚鼠卵巢的生长发育是否存在相互影响的关系。方法 将胚鼠卵巢分别移植至单侧卵巢摘除及去势雌鼠皮下，观察同时存在的自身卵巢对移植物生长发育有无影响，以及是否受移植物影响。结果 单摘移植的宿主卵巢黄体总体均数较高，差异（F=11.611,P=0.001）varian Tissues in Rats Haiyan Qin 1 Xuehui Huang 1 Fang Ma1 (1 Kunming Medical College. Add：No.295 Xichang Road, Kunming, Yunnan, China. Postal code: 650032) 【Abstract】Objective: To discuss whether follicles in situ ovaries and in grafts influence the growth and development of each other. Methods: we designed the experiment to investigate the simultaneous ovary remained wheter affects or is affected by grafted fetal ovarian tissue after transplantation of fetal ovarian tissue to recipients that retained one of in situ ovaries, or that received oophorotomy respectively. Result : Graft in recipients that had removed one of in situ ovaries growed and developed with more population mean of corepus luteum, the differences （F=11.611,P=0.001）between different groups are important. Graft in recipients that removed their own ovaries could grow and develop with all phases of follicles, and that remained one own ovary stopped continuing development，or Fibrosis even absorbed. Conclusion: Follicles in situ ovaries and in grafts compete dominance with each other. Follicles in ovaries of recipients evidently have more advantages than follicles in grafts. 【Key words】Fetal Ovarian tissue; heterotopic transplantation; Rat; Reproduction; Endocrine Secretion 胚胎卵巢组织处于无功能的未发育状态，其组织相容性抗原含量少，免疫原性低，是较好的移植供体来源。 以往关于胚胎鼠卵巢移植到成年鼠的研究，仅观察了移植物一方的生长，而未关注移植物对受者自身卵巢是否有影响。本研究将进一步研究二者的生长发育及其相互影响。 1 材料与方法 1.1 研究对象及分组 饲养室温20℃-28℃, 12h光照，12h黑暗。8-12周未交配体重在200-250g的雌性Sprague Dauley大鼠40只随机分组如下[实验动物生产许可证 SCXK（滇）2005-0008；实验动物使用许可证 SYXK（滇）2005-0004]： A 正常对照（n=8），仅行假手术； B 单侧卵巢摘除组（n=8）； C 单侧卵巢摘除+移植组（n=8）； D 去势组（n=8）； E 去势+移植组（n=8）； 饲养一周，经阴道脱落细胞涂片证实有正常动情周期者作为实验初选对象。 1.1.1 受者 如于造模（假手术、单侧卵巢摘除、去势手术）第4周，去势术后后大鼠性周期持续维持在动情间期、单侧卵巢摘除后阴道涂片提示仍有规律性周期者，入选为标准移植对象。 1.1.2 移植物 新鲜孕18-20天