硼替佐米对U87胶质瘤放射增敏及细胞周期分布的影响.docVIP

硼替佐米对U87胶质瘤放射增敏及细胞周期分布的影响 樊锐太 胡勇 刘俊启 张晶晶 陈昱丞 宋锐 顾浩（450052 郑州，郑州大学第一附属医院放疗科） [摘要] 目的 探讨硼替佐米(bortezomib BTZ)对放射治疗U87胶质瘤裸鼠移植瘤的增敏作用和细胞周期分布的影响。方法 建立裸鼠U87胶质瘤模型。造模成功的裸鼠随机分为组照射组BTZ）和联合组（RT+BTZ），裸鼠经8MeVβ线照射治疗,。结果 G0/G1期细胞比例下降（79.9% vs 66.0%，P 0.01），G2/M期细胞比例显著提高（3.2% vs16.4%，P 0.01）；BTZ联合RT对小鼠生存时间也有较大改善。结论G0/G1期进程阻滞，提高 G2/M期细胞比例，延长带瘤小鼠生存时间。 [关键词] 恶性脑胶质瘤；硼替佐米；放射治疗；放射增敏 [中图法分类号] [文献标志码] A Study of The Effect of radiosensitivity and Cell Cycle Distribution of Bortezomib on U87 glioma Fan Ruitai , Hu Yong ,Liu Junqi, Zhang Jingjing, Chen Yucheng, Song Rui,Gu Hao(Department Of Radiotherapy, First Affiliated Hospital of Zhengzhou University, Zhengzhou,Henan Province,450052,China) [Abstract] Objective To investigate the effect of bortezomib (BTZ) on malignant glioma radiosensitivity and cell cycle distribution in nude mice with U87 xenograft model. Methods The U87 xenograft model was established. All mices were assigned randomly divided into 4 groups:control group (CTRL), Radiation group (RT), bortezomib group(BTZ) and joint group(RT+BTZ). Nude mices were treated by 8MeV-β electron beam radiation. The joint group were given BTZ.Observe the effect of BTZ intraperitoneal injection on the inhibition rate , cell cycle distribution of tumor, tumor growth delay time (TGD), sensitizing enhancement ratio (SER), and compared survival time of nude mice in different groups. Results .The inhibition rate of RT+BTZ group was 46.5%,and superior to RT group（31.5%, F=25.6，P=0.000.0). The TGD of joint group was 59d vs 38d (RT group), and the sensitizing enhancement ratio of BTZ was 1.44. Showed that BTZ + RT make the sensitivity of radiotherapy alone 1.44 times improve. Combining BTZ with RT seemed to given that cells in G0/G1 decreased (79.9% vs 66.0％), P=0.005 0.01 and cells in G2/M increased (3.2％ vs 16.4％), P=0.00 0.05, significant difference was found between groups. Survival time of nude mice was extended in BTZ + RT group. Conclusion BTZ has obvious radios