nmda receptor subunit nr2b expression in cultured neural stem cells in vitro（nmda受体亚基nr2b表达在体外培养的神经干细胞）.docVIP

NMDA receptor subunit NR2B expression in cultured neural stem cells in vitro Author: Hu Zhonghao, Shanshan, Tie-Jun Xu [Keywords: neural stem cells; NMDA receptor subunit Unit 2B; Western blot Abstract: Objective To study the in vitro culture of rat neural stem cells (NSCs in N-methyl-D-aspartate (NMDA receptor subunit NR2B expression of separation from pregnant 18 to 19 days fetal rat hippocampus culture NM-DA, passages, identification of NSCs, immunocytochemistry, Western blot immunodetection passage NSCs NMDA receptor subunit NR2B expression results from pregnant 18 to 19 days fetal rat hippocampal NSCs can express The NSCs receptor subunit NR2B. conclusions were cultured in vitro expression of NMDA receptor subunit NR2B. Keywords: neural stem cells; NMDA receptor subunit 2B; Western blot analysis Abstract: Objective To investigate the expression of NMDA receptor subunit2B in the cultured neural stem cells (NSCs.Methods NSCs from the hippocampus of rats at embryonic day18-19were isolated, cultured, passaged and identi-fied.The expression of N-methyl-D -aspartate (NMDAreceptor subunit2B in the passaged NSCs was determined by im-munocytochemistry and Western blot.Results It was found that NSCs derived from the hippocampus of rats at embryonic day18-19could express NMDA receptor subunit2B.Conclusion NSCs can express NMDA receptor subunit2B in vitro . Keywords:: neural stem cells; NMDA receptor subunit2B; Western blot Since the 1990s, one after another from the mammalian central nervous system, especially the the adult brain periventricular room tube membrane lower dentate gyrus the particles lower, striatum, and other parts of neural stem cells isolated and cultured [1] ( neural stem cells, NSCs.NSCs the growth and differentiation of a variety of nerve growth factor and neurotrophic factor, which plays an important role of glutamate and its receptor. glutamate is one of the main body of the excitatory amino acid its excessive release through