about shenkangwan proliferation of cultured rat mesangial cells extracellular matrix secretion and cell cycle（关于shenkangwan体外培养的大鼠系膜细胞内增殖细胞外基质分泌和细胞周期）.docVIP

About Shenkangwan proliferation of cultured rat mesangial cells extracellular matrix secretion and cell cycle [Abstract] observed the Shenkangwan high glucose in vitro cultured rat mesangial cells (MC proliferation and secretion of extracellular matrix (ECM and cell cycle. [Methods] 16 male Wistar rats were randomly divided into four groups, normal serum group, captopril group (dose 5ng/kg, and Shenkangwan high dose of the low-dose group were 2.4,1.2 g / kg), fed with corresponding drugs 7d, drug serum separation and preparation to high glucose in vitro (30mmol/LGlu cultured MC were divided into five groups: high sugar model group (30mmol/LGlu), normal serum (volume fraction of 5% normal serum, Captopril group (5% serum and renal Pill Capoten the low-dose group (5% Shenkangwan high, low doses of serum, another low-sugar (10mmol/LGlu model group. cultured for 72h, using tetramethyl the azo bite salt (MTT assay of cell proliferation, double-antibody sandwich enzyme -linked immunosorbent assay (ELISA assay supernatant fibronectin protein (FN content, flow cytometry cell cycle. [Results] MC cultured in high glucose significantly proliferative, The supernatant FN content increased significantly, and the emergence of the cell cycle abnormalities, of Shenkangwan can inhibit MC proliferation, reduce the secretion of the FN, and Go / G phase cells increased significantly, reducing the proportion of cells with phase and G2 / M phase. [Conclusion] Shenkangwan the anomaly by adjusting the MC cell cycle inhibit high glucose-induced MC proliferation and ECM secretion. [Keywords] shenkang the bolus of Science diabetic nephropathy TCD therapy cell cycle cell cultured rat Diabetic nephropathy (diabetic nephropathy, DN diabetes (diabetes mellitus, in DM systemic microvascular complications, but also led to the death of patients with DM important reason for approximately 40% of type 1 DM and 5% to 10% of patients with type II DM can all structural damage the kidneys a DN.DM