乙肝病毒X蛋白地稳定表达对肝细胞凋亡和细胞周期地影响与机制.pdfVIP

  • 1
  • 0
  • 约8.25万字
  • 约 66页
  • 2017-09-05 发布于江苏
  • 举报

乙肝病毒X蛋白地稳定表达对肝细胞凋亡和细胞周期地影响与机制.pdf

Theeffectson andcell in linesstable apoptosiscyclehepatocyteby of BvirusX expressionhepatitis protein Abstract effectsof and Aim:To the B on virus(HBV)X investigate hepatitis geneapoptosis cell in lineHL一7702anddiscussthe mechanismsinthe cyclehepatocyte possible pathway. Methods:111ereconstituted wasestablished plasmidpcDNA3一X through recombinationDNA reconstituted and technique.11le plasmidpcDNA3一Xempty weretransfectedinto cellsand 0418tO vector HL-7702 selected pcDNA3 by constructtwoneweelllinewhichwerenamedHL.7702一HBxandHL.7702一con andWestern-blotwereusedtOconfirmthatHBVX respectively.RT-PCRanalysis of inHL一7702一HBxcells.Then andcell the geneexpressedsteadily apoptosiscycle twocellsweredetectedDNA andelectronic by ladder,flowcytometricanalysis inthesetwocellswere observation.Gene subsequently microscope expressions evaluated Human andcell someofresults by cyclegenearrays.And using apoptosis werefurtherconfirmedReal—timePCRandWestem—blot by analysis. and showedthata stablecelll

文档评论(0)

1亿VIP精品文档

相关文档