a new laccase biosensor for polyphenols determination茶多酚的新漆酶生物传感器的决心.pdfVIP

Sensors 2003, 3, 166-175 sensors ISSN 1424-8220 © 2003 by MDPI /sensors A New Laccase Biosensor for Polyphenols Determination * S.A.S.S. Gomes and M.J.F. Rebelo CECUL -Faculdade de Ciências da Universidade de Lisboa, Campo Grande, C 8, 1749-016 Lisboa. Tel: +351 21 Fax: +351 21 7500088. * Author to whom correspondence should be addressed. E-mail: mjrebelo@fc.ul.pt Received: 1 April 2003 / Accepted: 5 May 2003 / Published: 29 June 2003 Abstract: The relevance of polyphenols in human health is a well known fact. Prompted by that, very intensive research has been directed to get a method to detect them, which will improve the current ones. Laccase (p-diphenol:dioxygen oxidoreductase, EC ) is a multi-copper oxidase, which couples catalytic oxidation of phenolic substrates with four electron reduction of dioxygen to water [1] . A maximum catalytic response in oxygenated electrolyte was observed between 4.5 and 5.5 [2], while for pH 6.9 the laccase was found to be inactive [3]. We prepared a biosensor with laccase immobilised on a polyether sulphone membrane, at pH 4.5, which was applied at Universal Sensors base electrode. Reduction of the product of oxidation of several polyphenols, catalysed by laccase, was done at a potential for which the polyphenol of interest was found to respond. Reduction of catechol was found to occur at a potential of –200mV,