a plant natriuretic peptide-like molecule of the pathogen xanthomonas axonopodis pv. citri causes rapid changes in the proteome of its citrus host植物病原体的利钠peptide-like分子、黄axonopodis pv。.pdfVIP

Garavaglia et al. BMC Plant Biology 2010, 10:51 /1471-2229/10/51 RESEARCH ARTICLE Open Access A plant natriuretic peptide-like molecule of the pathogen Xanthomonas axonopodis pv. citri causes rapid changes in the proteome of its citrus host 1,2† 3† 1 1 1 3 Betiana S Garavaglia , Ludivine Thomas , Tamara Zimaro , Natalia Gottig , Lucas D Daurelio , Bongani Ndimba , 1 1* 3,4 Elena G Orellano , Jorgelina Ottado , Chris Gehring Abstract Background: Plant natriuretic peptides (PNPs) belong to a novel class of peptidic signaling molecules that share some structural similarity to the N-terminal domain of expansins and affect physiological processes such as water and ion homeostasis at nano-molar concentrations. The citrus pathogen Xanthomonas axonopodis pv. citri possesses a PNP-like peptide (XacPNP) uniquely present in this bacteria. Previously we observed that the expression of XacPNP is induced upon infection and that lesions produced in leaves infected with a XacPNP deletion mutant were more necrotic and lead to earlier bacterial cell death, suggesting that the plant-like bacterial PNP enables the plant pathogen to modify host responses in order to create conditions favorable to its own survival. Results: Here we measured chlorophyll fluorescence parameters and water potential of citrus leaves infiltrated with recombinant purified XacPNP and demonstrate that the peptide improves the physiological conditions of the tissue. Importantly, the proteomic analysis revealed that these responses are mirrored by rapid changes in the host proteome that include the up-regulation of Rubisco activase, ATP synthase CF1 a subunit, matu