frozen embryos implanted successful pregnancy a case report（冷冻胚胎移植成功的妊娠病例报告）.docVIP

Frozen embryos implanted successful pregnancy a case report : Yao Zhiwei Wing against the United States, the Perpetual Mei Hexiu Hong Meng Lifang [Keywords:] embryos frozen [Keywords:] of embryo freezing, in vitro fertilization, embryo transfer Implantation of embryo freezing technology is one of the assisted reproductive technology, it has the advantage to take more than one egg, embryo cryopreservation is not used to save if the patient first implant is unsuccessful, the next available after thawing of embryos for transplantation. no longer need the drug injection, and vaginal puncture oocytes. hospital reproductive center in 2003 to carry out the technology, and one of the patients receiving the treatment of a successful pregnancy in 2004, North of Shandong Province the first to accept the frozen embryo transfer successful cases are presented below. 1 clinical data 1.1 General Information By the Quality embryos of 10 patients, female, 35 years old, secondary infertility, in vitro fertilization and embryo transfer (IVFET) surgery in December 2003, COH 16 by in vitro fertilization, culture, transplantation 3, and the remaining 7 to be frozen after the failure of the IVFET cycle in September 2004, recovery of the frozen embryos, the selection of three implanted in the womb. 1.2 Methods Slow freezing fast fusion, the specific operation as follows. 1.2.1 Frozen: For frozen embryos has on the pretreatment, together with the frozen liquid into the straw from the low permeability to three different concentrations of hypertonic cryoprotectant solution, the program placed on computer-controlled freezing instrument within the program freezing. from the initial temperature of 20 ?C to -80 ?C temperature drop rate was maintained at 02 ~ 3 ℃ per minute to -80 ° C straws remove the set of liquid nitrogen and long-term save. 1.2.2 thaw: straws containing the embryos are removed from the liquid nitrogen and shaking for 40 s at room tempe