high performance liquid chromatography determination of imipenem - west division sodium content（高效液相色谱法测定imipenem -西司钠含量）.docVIP

High performance liquid chromatography determination of imipenem - West Division sodium content [Abstract] Imipenem - West Division buna spectrum antimicrobial activity of new beta-lactam antibiotics, both strong plant, beta-lactamase inhibition. HPLC chromatographic Asia amine imipenem - West Division buna, the method is reliable, simple and accurate. [Keywords:] West Division buna; high performance liquid chromatography Instruments and reagents Island of Tianjin LC2010A high performance liquid chromatography, Waters, Millipore vacuum pump, SB5200 ultrasonic instrument, the F13 pH meter, MultiSpec1501 Shimadzu UV spectrophotometer. Three batches of raw materials of CS, CS standard (purity 97.8%) provided by the city for Drug Control, and acetonitrile were of HPLC grade, ammonium phosphate, potassium dihydrogen phosphate, hydrochloric acid, 30% hydrogen peroxide, sodium hydroxide were of analytical grade. 2 Methods and Results 2.1 Column: Diamond C18 (4.6mm × 250mm, 5μm); mobile phase: acetonitrile: 0.1 mol / L, ammonium phosphate (precision to take ammonium phosphate 20.31mg, add 1000ml of water to dissolve and dilute to the mark with phosphoric acid adjusted to pH6 0.8) (30:70), flow rate 0.8ml/min, detection wavelength of 210nm, column temperature 50 ° C, the injection volume was 20μl. column efficiency and degree of asymmetry access CS control solution injection, theoretical plate number CS peak calculated for the 7070 injection precision test to take the CS control solution for injection 5-pin, peak area RSD of 0.008%, show that the system precision of CS reference standard for high temperature, oxidation, acids, alkalis, destruction trials to study its degradation products and the peak separation of selected chromatographic conditions, taking the above damage for the test solution injection, the results for effective separation of the main peak with the impurity peak, suggesting that oxidation and acid damage to produce more large impuriti