high performance liquid chromatography the rattus ear grass quercetin content（高效液相色谱的鼠耳草槲皮素含量）.docVIP

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2017-09-06 发布于浙江
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high performance liquid chromatography the rattus ear grass quercetin content（高效液相色谱的鼠耳草槲皮素含量）.doc

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high performance liquid chromatography the rattus ear grass quercetin content（高效液相色谱的鼠耳草槲皮素含量）

High Performance Liquid Chromatography the Rattus ear grass quercetin content [Keywords] HPLC Abstract: The purpose of the establishment of Rattus ear grass Determination of Quercetin method column: ODS Kromasil C18 column (4.6 mm × 150 mm, 5 μm, column temperature: 35 ° C; mobile phase: methanol 0. 4% phosphoric acid (volume ratio 45:55), flow rate: 1.0 mL / min, detection wavelength: 365 nm. Results Quercetin injection volume in the range of 0.005 ~ 0.045 μg good linear relationship (r = 0.9997) and the peak area recovery rate of 99.88%, RSD 0.58%. CONCLUSION The method is simple, reliable, and quality control can be used for the of Rattus ear grass and its preparations. Keywords: Rattus ear grass; quercetin; Determination of content; performance liquid chromatography Abstract: Objective To establish an HPLC method for content determination of quercetin in Hedyotis chrysoticha (Palib.) Merr. Methods HPLC method was carried on Kromasil C18 column using methanol0.4% phosphoric acid (45:55) as the mobile phase under the detection wavelength of 365 nm.Results The calibration curve for quercetin was linear in the range of 0.005-0.045μg. The average recovery was 99.88% with RSD 0.58%. Conclusions The method is simple, convenient and reliable for quality control of Hedyotis chrysoticha (Palib .) Merr. Keywords:: Hedyotis chrysoticha; HPLC; quercetin The Rattus ear grass the the the Rubiaceae plant Rattus Hedyotis Hedyotis chrysoticha the (Palib.) Merr. Dried or fresh grass in summer and fall closing, dried or fresh use. Origin: Anhui, Jiangsu, Zhejiang, Jiangxi, Fujian , Hunan, Guangdong, Guangxi, Yunnan and other provinces. spicy, slightly bitter, cool. Heat and dampness, stasis to stop bleeding, swelling detoxification Shanghai Chinese Materia Medica Standards (1994). According to the literature, Rattus ear grass contain alkaloids, iridoid terpenes, flavonoids and other chemical components, such as iso-mouse Li Su 3 - rutinoside, rutin [1]. repor