大体标本冰冻薄层断面切片机的研制.PDFVIP

第 23 卷第 11 期 第 　三 　军 　医 　大 　学 　学 　报 Vol. 23 , No. 11 　　　　 　　　　 1340 2001 年 11 月 ACTA 　ACADEMIAE 　MEDICINAE 　MILITARIS 　TERTIAE Nov. 2001 ( ) 论著 　　　 文章编号 2001 大体标本冰冻薄层断面切片机的研制 ( ) 高全胜 ,谭立文 ,应大君 　　第三军医大学基础医学部解剖学教研室 ,重庆 400038 　　提 　要 : 目的 　制备大体冰冻薄层断面标本 ,为三维重建提供丰富、准确的原始数据。方法 　在冰冻切片技术的基础上设计 和自制大体冰冻软组织切片机 ,改进了相关的冰冻技术 ,在冰盒内通过定标板埋设定标线 ,利用螺旋升降系统调节断层厚度。结果 ( ) 　断层厚度小于 0. 5 mm 本实验为 0. 865 mm 。并解决了冰冻标本的定标问题。结论 　该切片机操作简单、经济、实用 ,获得的图 像清晰、定位准确 ,且实验周期短 ,在生物组织三维重建中具有广阔的应用前景。 　　关键词 : 三维重建 ;定标 ;冰冻切片 　　中图法分类号 : R318. 6 ;R32234 　　 文献标识码 : A Preparation of cryomicrotome for thin crosssections of gross specimen GAO Quansheng , TAN Liwen , YING Dajun (Department of Anatomy , Third Military Medical University , Chongqing 400038 , china) 　　Abstract : Objective 　To prepare crosssections of frozen gross specimen to provide adequate original data for the threedimensional reestablish ment. Methods 　Based on the frozen section method , the frozen soft tissue microtome was designed and prepared. Some relevant frozen techniques were improved. The calibration line was buried in the ice box with the use of calibration board so that the thickness of the section could be adjusted by the spiral ascending and descending system. Results 　The thickness of the section was thinner than 0. 9 mm (0. 865 mm in our experiment) . The problem of calibration of the frozen specimen was solved. Conclusion 　The selfmade cryomicrootome is of simple operation , low cost and prac tical. By using this kind of cryomicrotome