药醋醅中产酸菌的产酸性能研究.docVIP

目 录 3 1. 材料与方法 3 1.1 试剂和样品 3 1.2 培养基 3 1.2.1基础发酵培养基 3 1.2.2 分离培养基 3 1.2.3 保藏培养基 3 1.2.4 产酸试验培养基 3 1.2.5 液体种子及发酵培养基 3 1.2.6 乙醇氧化培养基 4 1.2.7 生理生化试验培养基 4 1.2.8 生产性能测定用培养基 4 1.3 方法 4 1.3.1 分离纯化流程 4 1.3.2 分离方法 4 1. 3. 3 纯化 4 1. 3. 4 革兰染色 4 1. 3. 5 产酸定性试验 4 1.3.6 初筛 4 1.3.7 复筛 4 1.3.8 优势菌株的筛选 5 1.4 菌种初步鉴定 5 1. 4. 1 形态特征 5 1. 4. 2 培养特征 5 1. 4. 3 生理生化特征 5 2. 结果与分析 6 2. 1　分离筛选 6 2. 2　分离菌株的生物学特性 6 2.3 优势产酸菌菌株产酸性能的测定 7 2.3.1 优势产酸菌菌株 7 2.3.2 产酸速度及酒精转酸率 8 2.3.3 耐乙醇能力试验 9 2.3.4 耐温度能力试验 10 3. 讨 论 10 3.1 产酸菌的分离 10 3.2 优势产酸茵的产酸性能 11 3.3 药醋酿造中产酸菌的研究趋势 11 4. 结 论 11 参考文献 12 致谢 13 醋醅中产酸菌的分离与产酸性能研究 摘要： The acid properties and isolate of acid producing bacteria in vinegar Abstract:In the experiment,the research object were the normal and difierent batch,difierent ferment stage of solid-fermenters of Gansu factory.Separating acetobacter acetic strains adopted the changing colour circle method with bromocresol purple indicator.At last,12 acetobacter acetic strains were chosen from 52 created changing colour circle of single colony by purification again and again.Gram stain and qualitative experiments of producing acetate．In this experiment,by many sieving separates and produces acetate experimenting to 12 strains．five strains which have higher capability of producing acetate were got.Then they were mensurated three productions function ferments to experiment：acid-producing speed and alcohol conversion rate；alcohol degree resistance；thermostability．As a result，the two strains 2,5 were rapidly in acid-producing speed，and strain 5 had the highest alcohol degree resistance；the strain 4 thermostability was higher but the acid yield was medial.The ethanol tolerance and temperature resistant ability of strain 3 is weaker than other bacteria. Key words:acetobacter;isolate;Acid production performance 引 言 药醋其营养成分丰富,含有蛋白质、氨基酸、有机酸、无机物及各种糖类,并含有18 种以上的游离氨基酸,人体必需的8种氨基酸全部含有,营养、保健价值极高,在医学上具有重要的作用。醋性温味酸苦，具有开胃、养肝、散淤、止血、止痛、解毒等功效。1. 材料与方法 1.1 (第三天、第六天、第九天) 的药醋醅。 1.2 培养基 1.2.1基础发酵培养基[1]： 葡萄糖1%、