a dual-color luciferase assay system reveals circadian resetting of cultured fibroblasts by co-cultured adrenal glands一种既荧光素酶检测系统显示昼夜重置培养的成纤维细胞的培养的肾上腺.pdfVIP

A Dual-Color Luciferase Assay System Reveals Circadian Resetting of Cultured Fibroblasts by Co-Cultured Adrenal Glands 1¤a 2,3 4 1 ¤b Takako Noguchi , Masaaki Ikeda , Yoshihiro Ohmiya , Yoshihiro Nakajima * 1 Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan, 2 Molecular Clock Project, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Saitama, Japan, 3 Department of Physiology, Saitama Medical University, Moroyama, Saitama, Japan, 4 Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan Abstract In mammals, circadian rhythms of various organs and tissues are synchronized by pacemaker neurons in the suprachiasmatic nucleus (SCN) of the hypothalamus. Glucocorticoids released from the adrenal glands can synchronize circadian rhythms in other tissues. Many hormones show circadian rhythms in their plasma concentrations; however, whether organs outside the SCN can serve as master synchronizers to entrain circadian rhythms in target tissues is not well understood. To further delineate the function of the adrenal glands and the interactions of circadian rhythms in putative master synchronizing organs and their target tissues, here we report a simple co-culture system using a dual-color luciferase assay to monitor circadian rhythms separately in various explanted tissues and fibroblasts. In this system, circadian rhythms of organs and target cells were simultaneously tracked by the green-emitting beetle luciferase from Pyrearinus termitilluminans (ELuc) and the red-emittin