不同眼轮匝肌活动记录方法对豚鼠痕迹性眨眼条件反射建立的影响.docVIP

不同眼轮匝肌活动记录方法对豚鼠痕迹性眨眼条件反射建立的影响 杨 丽，胡 波，隋建峰（生理学教研室，第三军医大学，重庆400038） 提　要: 目的比较不同眼轮匝肌活动记录方法对豚鼠痕迹性眨眼条件反射建立的影响。方法 采用350 ms正弦波纯音和100 ms束状氧气流的配对刺激，训练豚鼠建立痕迹性眨眼条件反射。在此过程中，分别使用张力换能器和金属微电极记录眼轮匝肌活动，并使用我室编制的Matlab软件分析眨眼行为数据。 结果 两种记录方法条件下，豚鼠均能建立痕迹性眨眼条件反射，但应变片组动物条件眨眼反应的习得速率更快（P 0.05）。应变片组动物（P 0.05）结论相对于肌电图记录法，应变片记录法更适合联想学习研究。 中图法分类号：R331 Effect of different recording methods of orbicularis oculi muscle activity on the acquisition of trace eyeblink conditioning in guinea pigs Yang Li, Hu Bo, Fan Zheng-li, Huang Lü-shuai, Chen Hao, Sui Jian-feng (Department of Physiology, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China) Abstract: Objective To investigate the effect of different recording methods of orbicularis oculi muscle activity on the acquisition of trace eyeblink conditioning in guinea pigs. Methods A 350 ms tone conditioned stimulus (CS) was paired with a 100 ms corneal oxygen-puff unconditioned stimulus (US). The guinea pigs were trained for 10 consecutive sessions of trace eyeblink conditioning. During the experiments, a spring-return potentiometer or a metal microelectrode was used to detect the activity of orbicularis oculi muscle, respectively. Homemade software written under the Matlab environment was used to quantitatively analysis the behavioral data. Results Both groups of guinea pigs acquired the trace conditioned eyeblink responses (CRs). However, the animals using the spring-return potentiometer acquired the trace CRs more quickly compared with the animals using the microelectrode. In addition, the CR peak latency of the animals with potentiometer was significantly later than that of the animals with metal microelectrode on session 10. Conclusions Relative to the orbicularis oculi electromyogram, the spring- return potentiometer is more suitable for studying the neurobiological mechanisms underlying associative learning in guinea pigs. Key words: trace conditioning; eyeblink; orbicularis oculi muscle; guinea pig 痕迹